Binding‐Mediated Formation of Ribonucleoprotein Corona for Efficient Delivery and Control of CRISPR/Cas9

Abstract Protein coronae formed with nanoparticles confer several useful properties. However, the non‐specific nature of protein corona formation makes it difficult to deliver specific proteins for therapeutic applications. Herein, we report on construction a new type corona, termed binding‐mediated corona. This enables efficient and controllable delivery functional proteins, which is otherwise challenging conventional coronae. We show design ribonucleoprotein CRISPR/Cas9 system. Successful gene editing in human cell lines (Hela HEK293) demonstrates delivery, high stability, low cytotoxicity, well‐controlled activity Cas9‐guide RNA ribonucleoprotein. The strategy opens up opportunities delivery.